rabbit antibodies against ho 1  (Bioss)

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Calprotectin Is a Circulating Biomarker and Potential Therapeutic Target for Sarcopenia in Chronic Obstructive Pulmonary Disease

doi: 10.1002/jcsm.70196

Figure Lengend Snippet: Paquinimod attenuates the inflammatory response and oxidative stress in the cigarette smoke‐exposed mouse model. (A) The mRNA levels of inflammatory factors (TNF‐α, IL‐6, IL‐8, CXCL1, and IL1β) in skeletal muscle measured by qPCR. (B) TNF‐α and IL‐6 levels in different groups measured by ELISAs. (C) Levels of GSH, MDA, SOD2, and T‐AOC in the mouse Gast muscle. (D) Western blot analysis of the levels of antioxidant markers (Nrf2 and HO‐1) in each group of mice. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The primary antibodies used were a rabbit polyclonal antibody against GAPDH (1:1000; Cell Signalling Technology, 5174), a mouse monoclonal antibody against atrogin‐1 (1:1000; Santa Cruz Biotechnology, sc‐166 806), a mouse monoclonal antibody against MuRF1 (1:1000; Santa Cruz Biotechnology, sc‐398 608), a rabbit monoclonal antibody against Nrf2 (1:1000; Cell Signalling Technology, 12721), and a rabbit monoclonal antibody against HO‐1 (1:1000; Cell Signalling Technology, 26416).

Techniques: Western Blot

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/IJN.S466312

Figure Lengend Snippet: Preparation of RCDs/UA@Lipo-HAMA and schematic diagram of therapeutic mechanisms against adhesion. ( A ) Preparation of RCDs/UA@Lipo for dual-drug co-loading. ( B ) The mixture of RCDs/UA@Lipo and HAMA was precisely applied to the periphery of the sutured tendon model and photocured using 365 nm UV irradiation. This process solidified the HAMA, enabling a localized and sustained release of RCDs and UA. ( C ) RCDs/UA@Lipo-HAMA diminishes oxidative stress by activating Nrf2/HO-1, reducing CD68, and iNOS, and suppressing fibrosis proteins, thereby lessening inflammation and fibrosis, reducing tendon adhesion, and promoting healing.

Article Snippet: After permeabilized and blocked, the sections were incubated with rabbit anti-rat antibodies against Nrf2 (1:200; Cat# 80593-1-RR, Proteintech, USA), HO-1 (1:200; Cat#10701-1-AP, Proteintech, USA), CD68 (1:300; GB11067, Servicebio, China), and iNOS (1:200; Cat#18985-1-AP, Proteintech, USA) at 4°C overnight.

Techniques: Irradiation

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Chemical Evolution and Biological Evaluation of Natural Products for Efficient Therapy of Acute Lung Injury.

doi: 10.1002/advs.202305432

Figure Lengend Snippet: Figure 7. In vivo evaluation of compound 8b for ALI treatment. A) Wet/dry ratio. B) Total protein concentration in BALF. C) Number of white blood cells in BALF. D) MPO activity in lung tissues. E) The amount of TNF-𝛼in BALF. F) The amount of IL-1𝛽in BALF. G) The amount of IL-6 in BALF. H) ROS level in BALF. I) NO level in BALF. J) MDA level in lung tissues. K) SOD activity in lung tissues. L) 8b inhibited MAPK and NF-𝜅B phosphorylation in lung tissues. M) The protein levels of iNOS and COX2 were determined by Western blotting analysis. N) The protein level of Keap1 in lung tissues. O) The protein levels of Nrf2 and HO-1, GCLM were determined by Western blotting analysis. P) Representative images of lung H&E staining of Control, LPS, and 8b treatment groups. Data for (H–J) are normalized to respective controls. All data were presented as means ± SD (n = 6; * vs LPS, # vs Con, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; #### p < 0.0001; ns, no significant vs LPS).

Article Snippet: Antibodies against Keap1 (#8047S), HO-1 (#43966S), COX-2 (#12282S), iNOS (#13120S), p-p65 (#3033), p65 (#8242), p-p38 (#4511S), p38 (#8690S), p-ERK (#9101), ERK (#9102), p-JNK (#4668), JNK (#9258), Ubiquitin (#3936) and GAPDH (#2118) were purchased from Cell Signaling Technology (Massachusetts, USA).

Techniques: In Vivo, Protein Concentration, Activity Assay, Phospho-proteomics, Western Blot, Staining, Control

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Chemical Evolution and Biological Evaluation of Natural Products for Efficient Therapy of Acute Lung Injury.

doi: 10.1002/advs.202305432

Figure Lengend Snippet: Figure 8. 8b regulates Keap1/Nrf2 signaling pathway and alleviates oxidative stress and inflammation for ALI therapy.

Article Snippet: Antibodies against Keap1 (#8047S), HO-1 (#43966S), COX-2 (#12282S), iNOS (#13120S), p-p65 (#3033), p65 (#8242), p-p38 (#4511S), p38 (#8690S), p-ERK (#9101), ERK (#9102), p-JNK (#4668), JNK (#9258), Ubiquitin (#3936) and GAPDH (#2118) were purchased from Cell Signaling Technology (Massachusetts, USA).

Techniques: